A 13 kb resolution cosmid map of the 14 Mb fission yeast genome by nonrandom sequence-tagged site mapping
Article Abstract:
A 13 kb resolution cosmid map is presented for the 14 Mb fission yeast genome. This technique is advantageous over others since its utilization of nonrandom sequence-tagged site (STS) probes allows it to function not only as templates for DNA sequencing but also as a public data bank for accessing DNA at any region for gene cloning and mapping procedures. Other advantages are present. The technique is also an optimized version of random STS content mapping since it minimizes redundant work at every stage. However, various issues are discussed regarding the map's potential for mammalian genomes.
Publication Name: Cell
Subject: Biological sciences
ISSN: 0092-8674
Year: 1993
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Mis6, a fission yeast inner centromere protein, acts during G1/S and forms specialized chromatin required for equal segregation
Article Abstract:
The genes that mediated sister chromatid sepparation in centromeres were analyzed during mitosis in temperature-sensitive yeast mutants that carry a minichromosome. Mutants that lose the minichromosome at the permissive or semipermissive temperatures exhibited reduced viability due to alterations in the expression of mis6+. Disruptions in the chromosomal mis6+ gene also resulted in the unequal segregation of the chromosomes in yeast cells.
Publication Name: Cell
Subject: Biological sciences
ISSN: 0092-8674
Year: 1997
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Faithful anaphase is ensured by Mis4, a sister chromatid cohesion molecule required in S phase and not destroyed in G(sub)1 phase
Article Abstract:
A mutation of the fission yeast protein, Mis4, may form a link between chromatids in S phase that splits rather than being removed in anaphase. Research with Mis4 shows it is a different chromatid cohesion molecule, independent of cohesin, and can pass from yeast to human. Mis4 is neither essential for condensation and does not degrade in phase G(sub)1.
Publication Name: Genes & Development
Subject: Biological sciences
ISSN: 0890-9369
Year: 1998
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