Flow cytometric assessment of viability of lactic acid bacteria
Article Abstract:
Flow cytometry using carboxyfluorescein diacetate (cFDA) and the dye exclusion DNA binding probe TOTO-1 can distinguish between living and dead bacterial cells. In addition, cFDA labels the cells that are culturable whereas TOTO-1 labels the nonculturable cells.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2001
User Contributions:
Comment about this article or add new information about this topic:
A novel method for continuous determination of the intracellular pH in bacteria with the internally conjugated fluorescent probe 5 (and 6-)-carboxyfluorescein succinimidyl ester
Article Abstract:
The fluorescent probe 5 (and 6-)-carboxyfluorescein succinimidyl ester (cFSE) ratio method is a good technique for the measurement of intracellular pH (pH(sub in)) in bacteria. The difference in the fluorescent signals at pH-sensitive and pH-insensitive wavelengths gives a measure of the intracellular pH. The intracellular conjugation of cFSE prevents leakage of carboxyfluorescein. The method allows the investigation of pH(sub in) in bacteria under conditions of stress such as increase in temperature and the presence of detergents.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1996
User Contributions:
Comment about this article or add new information about this topic:
Rapid fluorescence assessment of the viability of stressed Lactococcus lactis
Article Abstract:
The fluorescent probes carboxyfluorescein and propidium iodide can be used to assess the viability of Lactococcus lactis following an environmental stress. Some of the stresses encountered by the bacterium include freezing and drying of starter cultures, low pH during fermentation, and low temperature and high salt concentration during cheese ripening.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1999
User Contributions:
Comment about this article or add new information about this topic:
- Abstracts: Osmotic stress leads to decreased intracellular pH of listeria monoytogenes as determined by fluorescence ratio-imaging microscopy
- Abstracts: Comparative genetic characterization of Listeria monocytogenes isolates from human and animal listeriosis cases
- Abstracts: Purification of Synechocystis sp. strain PCC6308 cyanophycin synthetase and its characterization with respect to substrate and primer specificity
- Abstracts: Differential expression of three alpha-galactosidase genes and a single beta-galactosidase gene from Aspergillus niger
- Abstracts: A general strategy for identification of S-layer genes in the Bacillus cereus group: molecular characterization of such a gene in bacillus thuringiensis subsp. galleriae NRRL 4045