Acquisition of a deliberately introduced phenol degradation operon, pheBA, by different indigenous Pseudomonas species
Article Abstract:
A research was conducted to investigate the acquisition of a deliberately released phenol degradation open by the indigenous microflora from river water polluted by phenolic compounds. P. putida and Escherichia coli were utilized as DNA recipient strains while pseudomonads were cultured at 30 degrees C. Results indicated that the expression of phe genes depended on the genetic background of the hosts. The dilution of the leachate water with river water contributed to the high efficiency of the natural biodegradation.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1997
User Contributions:
Comment about this article or add new information about this topic:
Small-scale DNA sample preparation method for field PCR detection of microbial cells and spores in soil
Article Abstract:
The construction of a small-scale, non-selective procedure for DNA sample preparation supporting rapid-in-the-field polymerase chain reaction analysis for detection of microbial spores and cells is described. Three methods capable of releasing genomic DNA from three microorganisms were chosen for cell and spore disruption techniques. The reagents and buffers used in the three methods were changed and the techniques are miniaturized to provide maximum DNA yields.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1998
User Contributions:
Comment about this article or add new information about this topic:
Generation of novel bacterial regulatory proteins that detect priority pollutant phenols
Article Abstract:
Researchers have created a Pseudomonas strain that can be used as a biosensor for phenols. This is done by placing a reporter gene under the control of a promoter that is induced by phenol or any other pollutant.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2000
User Contributions:
Comment about this article or add new information about this topic:
- Abstracts: Formation of fatty acid-degrading, anaerobic granules by defined species. Performance of anaerobic granules for degradation of pentachlorophenol
- Abstracts: Monooxygenase-mediated 1,2-dichloroethane degradation by Pseudomonas sp. strain DCA1. Interference of peptone and tyrosine with the lignin peroxidase assay
- Abstracts: Enzymatic mechanisms involved in phenanthrene degradation by the white rot fungus Pleurotus ostreatus. Degradation of the fluoroquinolone enrofloxacin by wood-rotting fungi
- Abstracts: Characterization of two novel propachlor degradation pathways in two species of soil bacteria. Propachlor removal by Pseudomonas strain GCH1 in an immobilized-cell system
- Abstracts: Anaerobic degradation of phthalate isomers by methanogenic consortia. The role of benzoate in anaerobic degradation of terephthalate