Aerobic degradation of 2,4,6-trinitrotoluene by Enterobacter cloacae PB2 and by pentaerytritol tetranitrate reductase
Article Abstract:
Research was conducted to examine the aerobic degradation of 2,4,6-trinitrotoluene (TNT) by Enterobacter cloacae PB2 and by pentaerytritol tetranitrate (PETN) reductase. The structural gene encoding PETN reductase was cloned and overexpressed in Escherichia coli. Results demonstrate that E. cloacae PB2 is also capable of growth with TNT as the sole source of nitrogen and that purified PETN reductase is capable of reducing the aromatic ring of TNT and causing the liberation of nitrite.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1998
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Metal accumulation and vanadium-induced multidrug resistance by environmental isolates of Escherichia hermannii and Enterobacter cloacae
Article Abstract:
Three bacterial strains which accumulate and are resistant to nickel and vanadium were isolated from contaminated soils surrounding an oil refinery. Two are Escherichia hermannii strains and is an Enterobacter cloacae strain. Growth in the presence of vanadium resulted in the production of multidrug resistance phenotypes of E. hermannii and E. cloacae. Incubation with vanadium altered the outer membrane profile (OMP) of E. hermannii but did not change the primary OMPs of E. cloacae.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1998
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Biodegradation of glycerol trinitrate and pentaerythritol tetranitrate by Agrobacterium radiobacter
Article Abstract:
Agrobacterium radiobacter stoichiometrically reduces glycerol trinitrate (GTN) to glycerol 1,3- and 1,2-dinitrate. The reaction shows regioselectivity for the formation of glycerol 1,3-dinitrate. The dinitrates are reduced to glycerol 1- and 2-mononitrates which are not degraded further. The bacteria also reduce pentaerythritol tetranitrate (PETN). The activity of the enzyme involved in the biodegradation of GTN and PETN increases in the presence of NADP.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1996
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