An NAD(P)H-nicotine blue oxidoreductase is part of the nicotine regulon and may protect Arthrobacter nicotinovorans from oxidative stress during nicotine catabolism

Article Abstract:

Transcriptional analysis and electronmobility shift assays have shown that the enzyme gene is expressed in a nicotine-dependent manner under the control of the transcriptional activator PmfR and hence a part of the nicotine regulon Arthrobacter nicotinovorans. The NAD(P)H-nicotine blue (quinone) oxidoreductase has prevented intracellular one-electron reductions of nicotine blue, leading to semiquinone radicals and potentially toxic reactive oxygen species.

Analysis, Oxidoreductases, Quinone, Quinones, Oxidative stress

---

Plasmids for nicotine-dependent and -independent gene expression in Arthrobacter nicotinovorans and other Arthrobacter species

Article Abstract:

The first inducible Arthrobacter overexpression systems is described based on the promoter/operator and the repressor of the 6-hydroxynicotine oxidase gene of Arthrobacter nicotinovorans and nicotine-dependent overproduction and affinity purification of recombinant proteins are presented. The system allows the production of complex enzymes and genetic complementation analysis in Arthrobacter species.

Science & research, Research, Genetic aspects, Genetic regulation, Gram-positive bacteria, Nicotinic receptors, Chemical properties

---

Catabolism of arylboronic acids by arthrobacter nicotinovorans strain PBA

Article Abstract:

Research demonstrates metabolic conversion of phenylboronic acid to phenol by Arthrobacter sp. strain PBA. The bacterium can also transform other arylboronic acids to monooxygenated products. Data indicate that the oxygen atom in the products is bound to the ring carbon atom previously bearing boronic acid substituent.

United States, Physiological aspects, Microbial metabolism, Microbiological synthesis, Catabolism

Similar abstracts:

• Abstracts: Distinct roles of c-Abl and Atm in oxidative stress response are mediated by protein kinase C delta. VHL-box and SOCS-box domains determine binding specificity for Cul2-Rbx1 and Cul5-Rbx2 modules of ubiquitin ligases
• Abstracts: A functional mobA gene for molybdopterin cytosine dinucleotide cofactor biosynthesis is required for activity and holoenzyme assembly of the heterotrimeric nicotine dehydrogenases of Arthrobacter nicotinovorans
• Abstracts: Prevalence and antimicrobial resistance of thermophilic Campylobacter spp. from cattle farms in Washington State
• Abstracts: Effects of dichloroethane isomers on the induction and activity of butane monooxygenase in the alkane-oxidizing bacterium Pseudomonas butanovora". part 2
• Abstracts: Inhibitory impact of bifidobacteria on the transfer of [beta]-lactam resistance among enterobacteriaceae in the gnotobiotic mouse digestive tract

This website is not affiliated with document authors or copyright owners. This page is provided for informational purposes only. Unintentional errors are possible.