An analysis of the instability kinetics of plasmid pHSG415 during continuous culture of Escherichia coli

Article Abstract:

The constant rate parameter model and the time-dependent parameter model help explain the influence of dilution rate on the instability kinetics of Escherichia coli RV308(pHSG415) at the time of glucose-restricted continuous culture. The plasmid segregation rate R and the variation in the specific growth rate between plasmid-bearing and plasmid-free cells, d-mu, that define the plasmid-host system are fitted according to the two models. While d-mu and R have values that do not vary with time in the first model, they are both depicted as functions that vary with time in the second model. The two models yield different results despite a good fit of the data. Several other plasmid instability models examined yield similar trajectories but define the rate parameters differently.

Models, Plasmids

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Escherichia coli periplasmic protein FepB binds ferrienterobactin

Article Abstract:

The periplasmic protein FepB binds to FeEnt, a part of the iron/B12 subsets of permease resulting in establishing FeEnt transport in the periplasmic permease part of transport systems. LppOmpA, a membrane localization vehicle, was used to establish FepB in the Escherichia coli outer membrane. It was found that Plasmid pTX700 developed under lac operator control encodes the tribrid protein LppOmpAFebB indicating that the LppOmpa localization vehicle can be used with periplasmic binding proteins.

Analysis, Carrier proteins, Transport proteins, Membranes (Biology)

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Biochemical genetics of a natural population of Escherichia coli: seasonal changes in alleles and haplotypes

Article Abstract:

An examination of 40 alleles and 159 haplogypes of E. coli strains from 16,000 individuals and other sources revealed the extent to which E. coli is able to select itself. The changes observed in the bacterium were large and significant, which leads credence to the selection theory. The samples of E. coli were obtained in May 1992, Jul 1992 and Aug 1992 to adequately measure any changes that would emerge over time. Changes were also attributed to possible diet changes.

Population genetics, Allelomorphism, Alleles, Haploidy

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