Analysis of errors in finished DNA sequences: the surfactin operon of Bacillus subtilis as an example
Article Abstract:
A comparative study of finished DNA sequences obtained from three laboratories helps estimate the nature and number of errors associated with the overlapping part of the DNA sequence. The errors are observed in secondary DNA structures and in compression in sequence ladders. A novel sequencing technique that decrease the error rate and the sequencing cost is discussed. Errors are also observed in the frameshift detection by computer programs.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 1995
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The Q15H mutation enables Crh, a Bacillus subtilis HPr-like protein, to carry out some regulatory HPr functions, but does not make it an effective phosphocarrier for sugar transport
Article Abstract:
Research was conducted to examine any changes in the catalytic and regulatory functions of catabolite repression HPr (Crh) protein by substituting glutamine-15 to a histidine residue. Results show that the modification, although it enables Crh carry out phosphorylation, it disables sugar transport function to Crh.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 1999
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Glycerol transport and phosphoenolpyruvate-dependent enzyme I- and HPr-catalysed phosphorylation of glycerol kinase in Thermus flavus
Article Abstract:
Research was conducted to demonstrate the mechanism of glycerol transport and phosphorylation of glycerol kinase in Thermus flavus. Results show glucose represses glycerol transport and metabolism, and phosphoenol-pyruvate dependent phosphorylate glycerol kinase.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 1999
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- Abstracts: Determination of a 12 kb nucleotide sequence around the 76 degree region of the Bacillus subtilis chromosome. Cloning and sequencing of a 40.6 kb segment in the 73 degrees-76 degrees region of Bacillu subtilis chromosome containing genes for trehalose metabolism and acetoin utilization
- Abstracts: A 12 kb nucleotide sequence containing the alanine dehydrogenase gene at 279 degrees on the Bacillus subtilis chromosome
- Abstracts: Engineering of plasmin-resistant forms of streptokinase and their production in Bacillus subtilis: streptokinase with longer functional half-life
- Abstracts: Importance of extracellular polymeric substances from Thiobacillus ferrooxidans for bioleaching. Sulfur chemistry in bacterial leaching of pyrite
- Abstracts: Analysis and dynamics of the chromosomal complements of wild sparkling-wine yeast strains. Karyotype rearrangements in a wine yeast strain by rad52-dependent and rad52-independent mechanisms