Analysis of proteins associated with growth of Bacteroides ovatus on the branched galactomannan guar gum
Article Abstract:
The protein profile of Bacteroides ovatus was analyzed to determine the enzyme patterns involved in the breakdown of guar gum. Growth on guar gum induced 12 soluble proteins and 20 membrane proteins. Among the proteins detected, galactomannanase I and II, membrane polypeptide o, and soluble proteins d and g were induced by both guar gum and glucose. Analysis of transposon-induced mutants revealed unchanged expression in the known degradative enzymes, while the derivative suppressor mutants showed some wild-type induced proteins as not limiting for growth on guar gum. Results indicate that regulation of guar gum utilization is complex.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1992
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Glucose toxicity and inability of Bacteroides ruminicola to regulate glucosetrasport and utilization
Article Abstract:
The effects of glucose and nitrogen limitation on cells of Bacteroides ruminicola B(sub 1)4 were studied. Under nitrogen-limiting conditions, glucose concentrations of 10 to 50 millimolar were toxic to cells. Toxicity was not dueto end product inhibition or nitrogen limitation. A glucose- resistant mutant transported glucose at a much lower rate and stored less polysaccharide than the wild type. These results indicate that B. ruminicola B(sub 1)4 is unable toregulate the transport and utilization of glucose under nitrogen-limiting conditions.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1992
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