Analysis of the enterohemorrhagic Escherichia coli O157 DNA region containing lambdoid phage gene p and Shiga-like toxin structural genes

Article Abstract:

A study was conducted to identify and sequence the p gene of the Shiga-like toxin(SLT)-II-converting the enterohemorrhagic Escherichia coli O157 phage 933W. The P gene sequences were used to find out the number of p genes in O157 and to study their linkage with distinct SLT genes and their restriction fragment length polymorphism. A polymerase chain reaction producing fragments hybridizing with both p and slt-I or slt-II sequences was developed.

Nucleotide sequence, Base sequence

---

Identification of unconventional intestinal pathogenic Escherichia coli isolates expressing intermediate virulence factor profiles by using a novel single-step multiplex PCR

Article Abstract:

One-step multiplex PCR (MPCR) is developed and evaluated for the differential identification of intestinal pathotypes of Escherichia coli. This shows that index virulence factors are not restricted to one pathogroup but instead can also occur in other pathotypes.

Science & research, Analysis, Physiological aspects, Genetic aspects, Polymerase chain reaction, Virulence (Microbiology), Nucleotide sequencing

---

Similar abstracts:

• Abstracts: Rectal carriage of enterohemorrhagic Escherichia coli O157 in slaughtered cattle. Shedding of Escherichia coli O157:H7 in calves is reduced by prior colonization with the homologous strain
• Abstracts: Genomic analysis of LexA binding reveals the permissive nature of the Escherichia coli genome and identifies unconventional target sites
• Abstracts: Identifying host sources of fecal pollution: Diversity of Escherichia coli in confined dairy and swine production system
• Abstracts: Display of bacterial lipase on the Escherichia coli cell surface by using FadL as an anchoring motif and use of the enzyme in enantioselective biocatalysis
• Abstracts: Shiga toxin 2e-producing Escherichia coli isolates from humans and pigs differ in their virulence profiles and interactions with intestinal epithelial cells

This website is not affiliated with document authors or copyright owners. This page is provided for informational purposes only. Unintentional errors are possible.