Aspartate carbamoyltransferase from a psychrophilic deep-sea bacterium, Vibrio strain 2693: properties of the enzyme, genetic organization and synthesis in Escherichia coli
Article Abstract:
The Vibrio strain 2693 genes for aspartate carbamoyltransferase (ATCase) were identified, sequenced, characterized and expressed in a heterologous expression system in Escherichia coli. The amino acid residues believed to be involved in the interactions between the subunits of ATCase are identical in Vibrio ATCase and E. coli ATCase indicating that two ATCases have similar tertiary and quaternary structures. The implications of these findings on the evolutionary relationships between Vibrionaceae and Enterobacteriaceae are discussed.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 1998
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Haemolysin production by strains of Verocytotoxin-producing Escherichia coli
Article Abstract:
The ability of Verocytotoxin-producing Escherichia coli (VTEC) to produce hemolysin is examined using 21 VTEC strains that hybridized with DNA probe CVD419. Results show an increase in hemolysin whenever blood agar plates are incubated under ideal atmospheric conditions. In addition, a rise in production is also observed when the strains were cultured on L-agar that contains ethylenediamine-di(0-hydroxyphenylacetic acid).
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 1998
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