Assessment of production conditions for efficient use of Escherichia coli in high-yield heterologous recombinant selenoprotein synthesis
Article Abstract:
The titrated levels of the selenoprotein mRNA under diverse growth conditions increase the yield of the over produced recombinant selenoprotein. The analysis illustrates the ability of Escherichia coli to upregulate the selenocysteine incorporation machinery on demand and shows that it can be used as cell factories for highly efficient production of heterologous selenoproteins such as rat thioredoxin reductase.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2004
User Contributions:
Comment about this article or add new information about this topic:
Plant transformation by coinoculation with a disarmed Agrobacterium tumefaciens strain and an Escherichia coli strain carrying mobilizable transgenes
Article Abstract:
Transformation of Nicotiana tabacum leaf explants was attempted with Escherichia coli as a DNA donor either alone or in combination with Agrobacterium tumefaciens. Escherichia coli donor strains were constructed harboring either the promiscuous IncP-type or the IncN-type conjugal transfer system and second plasmids containing the respective origins of transfer and plant-selectable markers.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2003
User Contributions:
Comment about this article or add new information about this topic:
Agrobacterium bioassay strain for ultrasensitive detection of N-acylhomoserine lactone-type quorum-sensing molecules: Detection of autoinducers in Mesorhizobium huakuii
Article Abstract:
An ultrasensitive bioassay system for the detection of N-acylhomoserine lactones (AHLs) was constructed in Agrobacterium tumefaciens by using the T7 expression system to overproduce the AHL receptor TraR. This strain detected many diverse AHLs, some at extremely low concentrations.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2003
User Contributions:
Comment about this article or add new information about this topic:
- Abstracts: Development of primers to O-antigen biosynthesis genes for specific detection of Escherichia coli O157 by PCR
- Abstracts: Flow cytometric analysis of the in situ accessibility of Escherichia coli 16S rRNA for fluorescently labeled oligonucleotide probes
- Abstracts: Antimicrobial resistance and virulence genes of Escherichia coli isolates from swine in Ontario. Mycotoxin fumonisin B(sub)1 increases intestinal colonization by pathogenic Escherichia coli in pigs
- Abstracts: Characterization of Shiga toxins gene (stx)-positive and intimin gene (eae)-positive Escherichia coli isolates from wastewater of slaughterhouses in France
- Abstracts: Characterization of an Escherichia coli O157:H7 plasmid O157 deletion mutant and its survival and persistence in cattle