Isolation of strong expression signals of Mycobacterium tuberculosis

Article Abstract:

Strong expression signals of Mycobacterium tuberculosis have been isolated using the natural fluorescence of the Aequoria victoria green fluorescent protein. Two of the promoters corresponded to proteins thought to be involved in magnesium and calcium utilization, very important for cell physiology and thus controlled by strong transctiption signals. The strength of the expression signals could be used for overexpression of foreign genes in mycobacteria to help with vaccine vector development and protein purification. Fluorescence-activated cell sorting (FACS) has been shown to offer a sensitive, efficient means to measure and select strong mycobacterial expression signals.

Australia, France, Statistical Data Included, Research, Methods, Usage, Reports, Causes of, Genetic aspects, Tuberculosis, Mycobacterium tuberculosis, Fluorescence, Bacterial genetics, Macrophages, Genetic regulation, Genetic transcription, Transcription (Genetics), Cytochemistry, Microbiological research, Bacteriology

---

Characerization of the expression and activity of the periplasmic nitrate reductase of Paracoccus Pantotrophus in chemostat cultures

Article Abstract:

Research shows that the expression of the nap gene, encoding the periplasmic nitrate reductase, is influenced by substrates and their reduction state. Data indicate that the highest level of expression and enzyme activity occurred in the order of butyrate greater than acetate greater than succinate.

Physiological aspects, Influence, Gene expression, Oxidation-reduction reaction, Oxidation-reduction reactions, Enzyme synthesis, Chemostat

---

Similar abstracts:

• Abstracts: Characterization of a novel type II restiction-modification system, Sth368I, encoded by the integrative element ICEst1 of Streptococcus thermophilus CNRZ368
• Abstracts: Isolation and characterization of draT mutants that have altered regulatory properties of dinitrogenase reductase ADP-ribosyltransferase in Rhodospirillum rubrum
• Abstracts: Structure of the ask-asd operon and formation of aspartokinase subunits in the cephamycin producer 'Amycolatopsis lactamdurans'
• Abstracts: Eubacterial arylamine N-acetyltransferases: identification and comparison of 18 members of the protein family with conserved active site cysteine, histidine and aspartate residues

This website is not affiliated with document authors or copyright owners. This page is provided for informational purposes only. Unintentional errors are possible.