Characterization of a highly repeated DNA sequence (SC1) from the arbuscular mycorrhizal fungus Scutellospora castanea and its detection in planta
Article Abstract:
The SC1 DNA sequence in the genome of the arbuscular mycorrhizal fungus Scutellospora castanea is 68% AT, with a molecular size of 1,202-bp. The sequence is arranged in tandem arrays containing a variable number of repeats. SC1 contains stop codons, perfect direct short repeats in the center and does not encode any protein. The sequence is used to prepare PCR primers to identify the fungi in mycorrhiza. SC1 is specific for detecting S. castanea as it does not amplify DNA from the spores or mycorrhizal roots containing Scutellospora species other than S. castanea.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1996
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Detection of an arbuscular mycorrhizal fungus in roots of different plant species with the PCR
Article Abstract:
The symbiotic association between arbuscular mycorrhizal (AM) and plant roots is prevalent in nature and the AM fungus Glomus intraradices from roots of five host colonized by this fungi was studied using PCR and AM taxon-specific primer, VANS1, and universal primer,NS21. The extent of root colonization was critical for detection. Storing the roots at -20 degree centigrades for more than two weeks removed the amplification of the 550-bp rDNA.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1995
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Intersporal genetic variation of Gigaspora margarita, a vesicular arbuscular mycorrhizal fungus, revealed by M13 Minisatellite-Primed PCR
Article Abstract:
The genetic variation between individual spores obtained from populations of Gigaspora margarita is revealed by minisatellite-primed PCR with the M13 core sequence. The evidence is provided by the clear variation of the fingerprints from analysis of the spores of G. margarita originating from single-spore cultures by M13 minisatellite-primed PCR. This establishes the heterokaryotic nature of the spores of other Glomales.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1997
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