Characterization of form variants of Xenorhabdus luminescens
Article Abstract:
Xenorhabdus spp. are entomopathogenic bacteria which forms symbiotic complexes with nematodes of the genera Steinernama and Heterorhabditis. Becauseof these complexes' potential as biological control agents, the form variants of X. luminescens XE-87.3 were characterized. Four form variants were isolated and found to differ in their pigmentation and antibiotic production. The four form variants could not be distinguished by DNA fingerprinting, indicating a clonal deriviation. However, a few differences were detected in their protein patterns, while their pathogenicity differed significantly.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1992
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Comparative analysis of antibiotic resistance, immunofluorescent colony staining, and a transgenic marker (bioluminescence) for monitoring the environmental fate of a rhizobacterium
Article Abstract:
Field releases of the wild-type plant growth-promoting rhizobacterium Pseudomonas fluorescens 89B-27, its bioluminescent derivative GEM-8 and a spontaneous rifampicin-resistant variant (R34) were established on cucumber. These were used to examine the efficacy of these marker systems for estimating the wild-type populations. Findings revealed that conventional marker systems may underestimate populations and hence the survival and colonization of genetically marked bacteria.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1997
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Polyclonal antisera to distinguish strains and form variants of Photorhabdus (Xenorhabdus) luminescens
Article Abstract:
Serological techniques use a new polyclonal antisera to differentiate between different strains and form variants of Photorhabdus luminescens. In the double-diffusion test antiserum 9226 exhibits different reactivity with different strains, revealing the serological difference between the strains. Cross-absorption increases the specificity of antisera, and primary and secondary forms of P. luminescens are discriminated using cross-absorbed antisera.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1995
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- Abstracts: Characterization and environmental regulation of outer membrane proteins in Xenorhabdus nematophilus. Purification and characterization of Xenorhabdicin, a phage tail-like bacteriocin, from the lysogenic strain F1 of Xenorhabdus nematophilus
- Abstracts: Comparison of ribotyping and randomly amplified polymorphic DNA PCR for characterization of Vibrio vulnificus