Cloning and nucleotide sequence analysis of gyrB of Bacillus cereus, B. thuringiensis, B. mycoides, and B. anthracis and their application to the detection of B. cereus in rice
Article Abstract:
The use of gyrB genes encoding the subunit B protein of DNA gyrase as targets of highly specific probes was explored in an effort to develop a rapid and reliable approach for differentiating Bacillus cereus from B thuringiensis. To this end, 1.2-kb fragments of the gyrB genes of B cereus, B anthracis, B. thuringiensis and B. mycoides were amplified, cloned and sequenced. A procedure for identifying the target organism in boiled rice regardless of its phenotypes, serotypes and virulence factors succeeded in detecting 0.24 CFU of B cereus cells per gram of boiled rice without extracting DNA.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1999
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Cloning of novel enterotoxin genes from Bacillus cereus and Bacillus thuringiensis
Article Abstract:
The pathogenic characteristics of Bacillus thuringiensis and Bacillus cereus were analyzed by the cloning and amino acid sequencing of toxin genes from Bacillus cereus FM1 strain and Bacillus thuringiensis israelensis. Analysis of the enterotoxin genes from the two Bacillus species indicated the presence of beta structures and consecutive Asn residues. Furthermore, the nucleotide sequences of the enterotoxin genes are similar in both Bacillus thuringiensis and Bacillus cereus.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1997
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Sequence diversity of the Bacillus thuringiensis and B. cereus sensu lato Flagellin (H antigen) protein: Comparison with H serotype diversity
Article Abstract:
The sequence diversity of the Bacillus thurinigiensis flagellin (H antigen [Hag]) protein is analyzed and compared with H serotype diversity along with other Bacillus ceres sensu lato species and strains. The amplification, cloning and determination of the nucleotide sequences of the flagellin allels from B. thuringiensis H serotypes and from B. thuringiensis-related species was reported in the Bacillus cereus senso lacto group.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2006
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