Colonization of wheat roots by an exopolysaccharide-producing Pantoea agglomerans strain and its effect on rhizosphere soil aggregation

Article Abstract:

Strain NAS206 from the rhizosphere of wheat Pantoea agglomerans was inoculated to examine the effect of bacterial secretion of an exopolysaccharide on rhizosphere soil physical properties. Colonization increased at the rhizoplane and in root-adhering soil after inoculation of wheat seedlings with the strain. The intense colonization of the wheat rhizosphere by these strains was associated with significant soil aggregation. Results indicated that P. agglomerans NAS206 can play a vital role in the regulation of water content of the rhizosphere by improving soil aggregation.

Microbial aggregation, Microbial polysaccharides, Wheat

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Direct ribosome isolation from soil to extract bacterial rRNA for community analysis

Article Abstract:

The combination of an adapted ribosome isolation method and the common RNA extraction method is effective in selective recovery of the natural microbial community rRNA in soils. Ultracentrifugation, based on the protein-like properties of the ribosomes, helps in recovering the ribosome pellet from the soluble soil ingredients. It is more difficult to isolate the complex protein-nucleic acid rRNA than the simpler rRNA molecules from pure cultures. Group-specific hybridization and oligonucleotide probes are used for yield comparison of the groups.

Usage, Nucleic acid hybridization, Ribosomal RNA, Centrifugation

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Variation of microbial communities in soil, rhizosphere, and rhizoplane in response to crop species, soil type, and crop development

Article Abstract:

The particular plant species growing in soil has the greatest impact on the microbial community in the rhizosphere. This was the conclusion of researchers who studied the impact of clover, bean, and alfalfa plants on soil bacteria. Plant development had a minor impact on soil bacteria.

Crops, Crops (Plants)

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