Conjugal transfer in Lactococcus lactis of a 68-kilobase-pair chromosomal fragment containing the structural gene for the peptide bacteriocin nisin
Article Abstract:
The nisin structural gene spaN was located by DNA hybridization and pulsed field gel electrophoresis in the chromosome of Lactococcus lactis subsp lactis DL11. Conjugal transfer of the spaN gene was possible, transferring along with it nisin resistance, ability to ferment sucrose and sensitivity to phage sk1. The transfer unit was found to be 68 kbp long, and inserted unstably in at least three different SmaI fragments. The transposon nature of the 68 kbp fragment may explain the various locations of spaN.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1992
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Rapid genomic fingerprinting of Lactococcus lactis strains by arbitrarily primed polymerase chain reaction with (super 32)P and fluorescent labels
Article Abstract:
The arbitrarily primed polymerase chain reaction (AP-PCR) procedure was used to obtain genomic fingerprints of different Lactococcus lactis strains. Using radioactive phosphorus, autoradiograms showed that AP-PCR was able to fingerprint the different strains of L. lactis, as well as show strain relationship and differentiation. Using fluorescent labels showed comparable patterns to those obtained on autoradiograms, although more AP-PCR products were resolved by the fluorescence method.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1992
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Identification of prophage genes expressed in lysogens of the Lactococcus lactis bacteriophage BK5-T
Article Abstract:
Analysis of the mRNA formed by lysogenic strains of the Lactococcus lactis bacteriophage BK5-T indicate that there are four main transcripts whose positions and sizes correspond to the open reading frames (ORFs). One of the transcripts is derived from the gene that encodes BK5-T integrase and the other from the gene encoding the BK5-T ORF that resembles the lambda cI repressor. A region of divergent putative promoters is identified from nucleotide sequence analysis.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1995
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