Deletions in the carboxyl-terminal region of Streptococcus gordonii glucosyltransferase affect cell-associated enzyme activity and sucrose-associated accumulation of growing cells

Article Abstract:

The CH2RPE and CH4RPE insertional mutant strains of Streptococcus gordonii Challis CH1 were utilized to determine the effects of mutations on the activity of the cell-associated glucosyltransferase (GTF). Changes in the carboxy-terminal region of GTF decreased enzyme activity without altering the levels of GTF proteins in the mutant strains. Insertional deletion of the 14 carboxy-terminal amino acids of GTF in CH2RPE and CH4RPE reduced by the levels of cell-associated GFT proteins due to reduced sucrose-associated accumulation.

Analysis, Microbial enzymes, Transferases, Microbial mutation

---

Effects of ionic and osmotic strength on the glucosyltransferase of Rhizobium meliloti responsible for cyclic beta-(1,2)-glucan biosynthesis

Article Abstract:

Research was conducted to investigate the effects of ionic and osmotic strength on the glucosyltransferase of Rhizobium meliloti responsible for cyclic beta-(1,2)-glucan biosynthesis. The regulation of the biosynthesis of these glucans were examine in vitro with membrane preparations from R meliloti. Results indicate that NdvA and the C terminus of NdvB are not essential for osmotic regulation of cyclic beta-(1,2)-glucan biosynthesis.

Fluid-electrolyte balance, Biosynthesis, Osmoregulation, Glucans, Rhizobium meliloti

---

Mutagenesis of Asp-569 of glucosyltransferase I glucansucrase modulates glucan and oligosaccharide synthesis

Article Abstract:

Asp-569 of glucosyltransferase I glucansucrase modulates glucan and oligosaccharide synthesis by its mutagenesis. Engineering the equivalent position in glucansucrases is potentially a way to increase the range of oligosaccharides synthesized.

United Kingdom, France, Bacteria, Oligosaccharides, Protein engineering, Mutagenesis

Similar abstracts:

• Abstracts: Enhanced (+)-catechin transglucosylating activity of Streptococcus mutans GS-5 glucosyltransferase-D due to fructose removal
• Abstracts: Pharmacogenetics of cancer therapy: getting personal. Epigenetic regulation of human gamma-glutamyl hydrolase activity in acute lymphoblastic leukemia cells
• Abstracts: DNA variation in a 5-Mb region of the X chromosome and estimates of sex-specific/type-specific mutation rates
• Abstracts: IdiA, a 34 kDa protein in the cyanobacteria Synechococcus sp. strains PCC 6301 and PCC 7942, is required for growth under iron and manganese limitations
• Abstracts: Solution structure of the link module: a hyaluronan-binding domain involved in extracellular matrix stability and cell migration

This website is not affiliated with document authors or copyright owners. This page is provided for informational purposes only. Unintentional errors are possible.