Determination of active marine bacterioplankton: a comparison of universal 16S rRNA probes, autoradiography, and nucleoid staining
Article Abstract:
Several techniques for the assessment of bacterial numbers and activity in marine waters were compared utilizing samples from a number of marine environments, from aged offshore seawater to rich harbor water. Samples were simultaneously tested for binding to a fluorescently labeled universal 16S rRNA probe; 3H-labeled amino acid uptake via autoradiography; nucleoid-containing bacterial numbers by modified 4',6-diamidino-2-phenylindole staining; staining with 5-cyano-2,3-ditolyl tetrazolium chloride; and total bacterial counts taken as reference.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1997
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Prevalence of the chloroflexi-related SAR202 bacterioplakton cluster throughout the mesopelagic zone and Deep Ocean
Article Abstract:
Newly identified SAR202-related 16S rRNA gene sequences are used to further resolve the phylogeny of the cluster and to design taxon-specific oligonucleotide probes for fluorescence in situ hybridization. On average, members of the SAR202 group accounted for 10.2 percent of all DNA-containing bacterioplankton between 500 and 4,000m.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2004
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Richness and diversity of bacterioplankton species along an estuarine gradient in Moreton Bay, Australia
Article Abstract:
Moreton Bay, Australia, consists of vast bacterioplankton community. This community is examined using rRNA intergenic spacer analysis, the results of which are discussed.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2004
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