Diversity and biocatalytic potential of epoxide hydrolases identified by genome analysis
Article Abstract:
Epoxide hydrolases plays an important role in the biodegradation of organic compounds and are potentially useful in enantioselective biocatalysis and analysis of various genomic databases reveals that about 20% of sequenced organisms contain one or more putative epoxide hydrolase genes. The results show that the expression of epoxide hydrolase genes that are detected by analyses of genomic databases is a useful strategy for obtaining new biocatalysts.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2006
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Trigonopsis variabilis D-amino acid oxidase: Control of protein quality and opportunities for biocatalysis through production in Escherichia coli
Article Abstract:
The flavoenzymes D-amino acid oxidases (DAOs) are used in the industrial conversion of cephalosporin C into glutaryl-7-aminocephalosporanic acid in the yeast Trigonopsis variabili. The recombinant TvDAO is analyzed for the catalycity in the Escherichia coli strains and show reduced toxicity in the microorganism.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2007
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Directed evolution of a thermostable phosphite dehydrogenase for NAD(P)H regeneration
Article Abstract:
An enzymatic system based on phosphite dehydrogenase from Pseudomonas stutzeri is developed to regenerate the reduced nicotinamide cofactors NADH and NADPH. Results would enable to reach the goal of developing a low-cost, highly efficient biocatalyst for NAD(P)H regeneration.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2005
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