Effect of promoter modification on mosquitocidal cryIVB gene expression in Synechococcus sp. strain PCC 7942
Article Abstract:
The effect of promoter modification on the expression of the mosquitocidal Bacillus thuringiensis subsp. israelensis cryIVB gene used to change Synechococcus sp. strain PCC 7942 when analyzed showed that maximal transcript and protein abundance were attained by the addition of the lacZ promoter upstream of the cryIVB sequence. While substituting the endogenous corresponding Bacillus sequences with the Synechococcus petF1 promoter, the ribosome binding site and the initiation codon caused an increased expression of the cryIVB gene, relative to the expression obtained with the Bacillus promoter.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1993
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Cloning and expression of a novel toxin gene from Bacillus thuringiensis subsp. jegathesan encoding a highly mosquitocidal protein
Article Abstract:
The Bacillus thuringiensis subsp. jegathesan cry11B gene encodes the Cry11B protein which is similar to the mosquitocidal toxin Cry11A from B. thuringiensis subsp. israelensis. The transcription of the gene, cloned using an oligonucleotide probe, involves two promoters. Purified Cry11B inclusions produced by cry11B expression in a nontoxic B. thuringiensis host are highly toxic for larvae of different mosquito species. The activity of Cry11B is more than that of Cry11A and similar to that of the wild-type B. thuringiensis inclusions.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1995
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Ethanol synthesis by genetic engineering in cyanobacteria
Article Abstract:
Research was conducted to examine ethanol synthesis by genetic engineering in cyanobacteria. New genes were introduced into a cyanobacterium to create a novel pathway for fixed carbon utilization that leads to the synthesis of ethanol. Zymomonas mobilis pdc and adh genes were cloned into a shuttle vector and utilized to transform the Synechococcus spp. strain. Results suggest that the production of ethanol by cyanobacteria is a potential method for the bioconversion of solar energy and CO2 into a valuable resource.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1999
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