Microvariation artifacts introduced by PCR and cloning of closely related 16S rRNA gene sequences
Article Abstract:
Research is presented on tracking artifactual sequence variation sources within 16S rRNA clone libraries. A polymerase chain reaction method was applied to a defined template mixture containing seven 16S-rRNA clones that are closely related; artifact sources found included a mutational hot spot, polymerase errors and heteroduplex and chimera cloning.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2001
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Effect of toxic metals on indigenous soil beta-subgroup proteobacterium ammonia oxidizer community structure and protection against toxicity by inoculated metal-resistant bacteria
Article Abstract:
The effect of treating soil with a mixture of toxic metals on the population structure and size of the ammonia oxidizers that are members of the beta subgroup of the Proteobacteria family has been studied. These metals are cadmium, cobalt, cesium, strontium and chlorides. The addition of five strains of metal-resistant heterotrophic bacteria to the soil has also been examined. Results revealed that the addition of the metals brought specific changes in the community structure of the ammonia oxidizers which were not seen with the metal-resistant bacteria.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1999
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Molecular analysis of ammonia-oxidizing bacteria of the beta subdivision of the class Proteobacteria in compost and composted materials
Article Abstract:
A molecular analysis of ammonia-oxidizing bacteria of the beta subdivision of the class Proteobacteria in compost and composted materials was conducted. The objective was to examine the distribution and community composition of the oxidizers in different types of composting materials by amplifying 16S ribosomal DNA and 16S rRNA using PCR and reverse transcriptase PCR, respectively. Results revealed the presence of nucleic acids from nitrifying bacteria in the materials which may have substantial implications for compost processing and storage.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1999
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