Enumeration of phenanthrene-degrading bacteria by overlayer technique and its use in evaluation of petroleum-contaminated sites
Article Abstract:
The enumeration of phenanthrene-degrading bacteria is made easier by a new technique which involves adding the bacteria to agarose containing fine particles of phenanthrene and pouring this mixture onto a solidified agar underlayer. Counting the relevant colonies becomes easier due to the visible halo of phenanthrene disappearance around them. Applying this technique in the quantification of phenanthrene-degrading bacteria to various soils revealed that samples from soil contaminated with petroleum had more of these kinds of bacteria. Sites contaminated with phenanthrene-containing compounds had the most numerous colonies.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1992
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Enumeration and detection of anaerobic ferrous iron-oxidizing, nitrate-reducing bacteria from diverse European sediments
Article Abstract:
A study was conducted to analyze the presence of anaerobic ferrous iron-oxidizing, nitrate-reducing bacteria in various European freshwater sediments. The number of prokaryotic cells were determined using the DNA-intercalating, blue fluorescent dye 4',6'-diamidino-2-phenylindole while ferrous iron concentrations were analyzed photometrically after chelation. Results indicated that enzymes involved in the anaerobic oxidation of ferrous iron can be utilized to effectively determine microbiological activities.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1998
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Seasonal dynamics of bacterial colonization of cotton fiber and effects of moisture on growth of bacteria within the cotton ball
Article Abstract:
The seasonal pattern of bacterial cotton fiber colonization was investigated. Dilution pour plating was used for the determination of the bacterial numbers of fiber samples while a modified dilution pour plating method was used for the characterization of gram-negative bacteria. Data were subjected to variance analysis and t tests. The growth of bacteria due to the rapid colonization of fiber was demonstrated.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1993
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