Expression of an Aspergillus niger phytase gene (phyA) in Saccharomyces cerevisiae
Article Abstract:
The Aspergillus niger phytase gene (phyA gene) was expressed as an active, extracellular phytase in Saccharomyces cerevisiae and its thermostability affected by glycosylation. This was found in a study that sought to determine if S cerevisiae was able to express functional phyA phytase and to investigate the effects of glycosylation on the activity and thermostability of the phytase. A 1.4-kb DNA fragment containing the coding region of the phyA gene was inserted into the expression vector pYES2, with the yield of extracellular phytase activity affected by the signal peptide and the medium composition. Deglycosylation of the phytase led to losses of 9% of its activity and 40% of its thermostability.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1999
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Molecular characterization and expression of a phytase gene from the thermophilic fungus Thermomyces lanuginosus
Article Abstract:
The phytase gene from the thermophilic fungus Thermomyces lanuginosus was identified, cloned and the recombinant gene product was molecularly characterized. Results demonstrate that T. lanuginosus performs at 79% of the Aspergillus niger phytase turnover number while the T. phytase operates at 67%-enhanced catalytic efficiency than the A. niger enzyme at 60 degrees C and over an expanded pH range.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1998
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Purification, characterization, and heterologous expression in Fusarium venenatum of a novel serine carboxypeptidase from Aspergillus oryzae
Article Abstract:
Researchers describe the properties of a serine carboxypeptidase from Aspergillus oryzae. Carboxypeptidases are used in food processing to hydrolyze food proteins. Unlike other proteases used for this purpose, they do not produce a bitter taste and in fact may boost the natural flavor of foods.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1999
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