In situ reverse transcription-PCR for monitoring gene expression in individual Methanosarcina mazei S-6 cells
Article Abstract:
Research has been conducted on the Methanosarcina mazei mRNA. The reverse transcription-PCR protocol to detect this mRNA is analyzed.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2000
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Expression of nifH genes in natural microbial assemblages in Lake George, New York, detected by reverse transcriptase PCR
Article Abstract:
Research has been conducted on the nitrogenase genes. The use of the reverse transcriptase polymerase chain reaction method in detecting the expression of these genes in a meso-oligotrophic lake is discussed.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2000
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Visualization of specific gene expression in individual Salmonella typhimurium cells by in situ PCR
Article Abstract:
The expression of the lac gene in Salmonella typhimurium was monitored using a modified polymerase chain reaction (PCR) technique. The lac messenger RNA levels was approximated using gene activity measures at the population level. This technique will provide researchers an opportunity to investigate specific gene expression in bacteria as well as determine bacterial spatial distribution in communities in natural habitats.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1997
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