Flow cytometry detection of infectious rotaviruses in environmental and clinical samples
Article Abstract:
An indirect immunofluorescence and flow cytometry (IIF-FC) method was developed to identify infectious rotaviruses from fecal or water samples. CaCO2 cell-line and ulterior FC were utilized to recognize infected cells because of the cell line's sensitivity to rotavirus infection. Acetone, methanol and formalin combinations were used as fixatives because of their ability to provide superior specific/signal background ratio. The technique offers several advantages such as reproducibility and the ability to process large number of samples.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1998
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Development, evaluation, and standardization of a real-time TaqMan reverse transcription-PCR assay for quantification of hepatitis A virus in clinical and shellfish samples
Article Abstract:
A standardized real-time reverse transcription-PCR (RT-PCR) assay was developed for an accurate estimation of the number of genome copies of hepatitis A virus (HAV) in clinical and shellfish samples. The best performance in terms of sensitivity and reproducibility was achieved by a one-step TaqMan RT-PCR, with the sensitivity enabling the detection of 0.05 infectious unit and 10 copies of a single-stranded RNA (ssRNA) synthetic transcript and the test proved to be highly specific after a broad panel of enteric viruses was tested.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2006
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Development and evaluation of a broadly reactive TaqMan assay for rapid detection of hepatitis A virus
Article Abstract:
Primers and a TaqMan probe for the 5' -untranslated region (UTR) of the hepatitis A virus (HAV) genome were designed and evaluated. The assay detected 0.5 infectious units of HAV and 40 copies of a synthetic transcript and provided an important screening tool for rapid quantitative HAV detection in clinical or environmental samples.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2005
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- Abstracts: Detection of diverse new Francisella-like bacteria in environmental samples. Acidobacteria phylum sequences in uranium-contaminated subsurface sediments greatly expand the known diversity within the phylum
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