Relationship between glycolysis and exopolysaccharide biosynthesis in Lactococcus lactis
Article Abstract:
Research on Lactococus lactis has produced clues for methods to increase exopolysaccharide (EPS) production via genetic manipulation. Nuclear magnetic resonance was used to determine phosphorylated intermediate concentrations in cell wall and EPS biosynthetic pathways; metabolic targets include an increase in pool size of the lipid carrier and an enhancement of EPS precursor production flux.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2001
User Contributions:
Comment about this article or add new information about this topic:
Functional analysis of the Lactococcus lactis galU and galE genes and their impact on sugar mucleotide and exopolysaccharide biosynthesis
Article Abstract:
Overexpression of the UPD-glucose pyrophosphorylase (galU) gene of Lactococcus lactis MG1363 under control of the nisA promoter causes an increase in GalU activity and increases in UDP-glucose and UDP-galactose. UDP-glucose and UDP-galactose are precursors of exopolysaccharides.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2001
User Contributions:
Comment about this article or add new information about this topic:
Stabilization of enzymes against thermal stress and freeze-drying by mannosylglycerate
Article Abstract:
A study was conducted on the impact of mannosylglycerate on the protection against freeze-drying of enzymes taken from thermophilic, mesophilic and hyperthermophilic sources. Results show that 2-0-Besta-mannosylglycerate, which accumulates in certain thermophilic organisms, is effective in stabilizing enzymes. Results also indicate the potential biotechnological applications of the solute.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1997
User Contributions:
Comment about this article or add new information about this topic:
- Abstracts: Leaky Lactococcus cultures that externalize enzymes and antigens independently of culture lysis and secretion and export pathways
- Abstracts: Imbalance of leucine flux in Lactococcus lactis and its use for the isolation of diacetyl-overproducing strains
- Abstracts: Synthesis of lactococcin 972, a bacteriocin produced by Lactococcus lactis IPLA 972, depends on the expression of a plasmid-encoded bicistronic operon
- Abstracts: Molecular analyses of the methane-oxidizing microbial community in rice field soil by targeting the genes of the 16S rRNA, particulate methane monooxygenase, and methanol dehydrogenase
- Abstracts: Heterologous coproduction of enterocin A and pediocin PA-1 by Lactococcus lactis: detection by specific peptide-directed antibodies