Distribution of 14C-labelled carbon from glucose and glutamate during anaerobic growth of Saccharomyces cerevisiae
Article Abstract:
A study was conducted to analyze carbon distribution from glucose and glutamate using anaerobically grown Saccharomyces cerevisiae. A Chemap CMF200 bioreactor equipment was utilized to prepare cultures while the biomass concentration was determined by OD(sub 610) measurements. Results showed that the carbon flow was characterized by two separate paths. Findings also indicated that only parts of the tricarboxylic acid cycle were utilized when glutamate was in the nitrogen source.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 1998
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The Nha1 antiporter of Saccharomyces cerevisiae mediates sodium and potassium efflux
Article Abstract:
Deletion of the NHA1 gene of Saccharomyces cerevisiae wild type strains, lead to higher sensitivity to both K+ and Na+ at acidic pH. High K+ and Na+ efflux seen on alkalinization of the cytoplasm suggests a role for Nha1p in the regulation of intracellular pH. The Nha1 alkalication antiporter is found to be responsible for growth on high concentrations of KC1 and NaC1.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 1998
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