High-frequency transfer of a naturally occurring chromosomal tetracycline resistance element in the ruminal anaerobe Butyrivibria fibrisolvens

Article Abstract:

Microbiological research has established the presence of a transmissible chromosomal element with tetracycline resistance in the obligately anaerobic rumen bacterial species Butyrivibrio fibrisolvens. Pulsed-field gel electrophoretic analysis demonstrates the transconjugant's altered chromosomal band profile. Evidence suggests that a distantly related Tc(super r) genetic determinant is transferred.

Tetracyclines, Rumen, Rumen microbiology, Transfer factor (Immunology), Transfer factor

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Three multidomain esterases from the cellulolytic rumen anaerobe Ruminococcus flavefaciens 17 that carry divergent dockerin sequences

Article Abstract:

Results demonstrate that all three esterase domains share amino acid sequence identity between them, exhibit dockerin-like regions, and catalytic domains. Data further indicate that dockerin from two of the esterases differ significantly and perhaps represent distinct specificities to dockerin.

United Kingdom, United States, Statistical Data Included, Physiological aspects, Esterases, Enzymes, Microbial metabolism, Amino acids, Enzyme structure-activity relationships, Amino acid structure-activity relationships

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EndB, a multidomain family 44 cellulase from Ruminococcus flavefaciens 17, binds to cellulose via a novel celluloase-binding module and to another R. flavefaciens protein via a dockerin domain

Article Abstract:

The properties of the 808-amino acid cellulase produced by the endB gene in Ruminococcus flavefaciens 17 are described. The EndB protein may bind to celluloase via a cellulose-binding module or via its involvement in a cellulosome-like multienzyme complex.

Microbiology, Microbial enzymes, Cellulose

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