Histidine phosphorylation of P-selection upon stimulation of human platelets: a novel pathway for activation-dependent signal transduction
Article Abstract:
Phosphorylation after platelet stimulation with thrombin or collagen produces phosphohistidine at histidines on the cytoplasmic tail of P-selectin.The two-component systems in prokaryotes which regulates significant physiological functions are marked by transient phosphorylation. But related events have not been involved in signal transduction in mammalian cells. To investigate histidine phosphorylation during stimulation of human cells, activated platelets were examined for the creation of protein phosphohistidine in a model system using P-selectin.
Publication Name: Cell
Subject: Biological sciences
ISSN: 0092-8674
Year: 1995
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Cell cycle-dependent polar localization of an essential bacterial histidine kinase that controls DNA replication and cell division
Article Abstract:
A study was conducted to analyze CckA, an essential histidine kinase promoting CtrA activation by phosphorylation. The Beckton Dickinson FACStar Plus machine was utilized to prepare flow cytometry samples. Ultraviolet-mutagenized CB15N cells were plated on soft agar supporting a bacteriophage. Experimental results indicated that dynamic changes in cellular location of critical signal proteins promote a unique mechanism for the control of the prokaryote cell cycle.
Publication Name: Cell
Subject: Biological sciences
ISSN: 0092-8674
Year: 1999
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Structure of CheA, a signal-transducing histidine kinase
Article Abstract:
A study was conducted to analyze the structure of CheA, a signal-transducing histidine kinase. Patterson map analysis was carried out to determine the mercury atoms positions of derivatized CheA. Experimental results indicated that CheA supports the nucleophilic histidine and activating glutamate for phosphotransfer. Findings also showed that relative domain motions connect receptor response to kinase activity.
Publication Name: Cell
Subject: Biological sciences
ISSN: 0092-8674
Year: 1999
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