Identification of vegetative proteins for a two-dimensional protein index of Bacillus subtilis
Article Abstract:
The vegetative proteins of Bacillus subtilis were characterized by the construction of two-dimensional (2-D) protein gels from crude extracts of the microbe. The 2-D gel electrophoresis of Bacillus subtilis extracts indicated the role of osmotic stress and glucose starvation in modulating the synthesis of enzymes involved in glycolysis and amino acid metabolism. Furthermore, 2-D gel electrophoresis of Bacillus subtilis crude extracts permitted the identification and separation of 600 distinct protein spots.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 1997
User Contributions:
Comment about this article or add new information about this topic:
Specific and general stress proteins in Bacillus subtilis: a two-dimensional protein electrophoresis study
Article Abstract:
The effects of ethanol, heat shock and salt stress on the protein profile of Bacillus subtilis were analyzed by high resolution two-dimensional (2-D) polyacrylamide gel electrophoresis. High resolution 2-D polyacrylamide gel electrophoretic analysis of Bacillus subtilis stress proteins indicated the presence of stress-specific proteins that were triggered by ethanol, heat shock and salt stress. Furthermore, general stress proteins were also activated by general stress induction mechanisms.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 1997
User Contributions:
Comment about this article or add new information about this topic:
Phosphate-starvation-inducible proteins in Bacillus subtilis; a two-dimensional gel electrophoresis study
Article Abstract:
The phosphate-starvation-inducible or Psi proteins of Bacillus subtilis (Bs)strain 168 was analyzed by two-dimensional gel electrophoresis. Analysis of Psi protein products from wild-type and phoR Bs mutants indicated the presence of two classes of proteins. Furthermore, the Class I Psi proteins are Pho regulons that exhibited PhoP/R-dependence while the class II PhoR-independent proteins are induced by glucose starvation.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 1996
User Contributions:
Comment about this article or add new information about this topic:
- Abstracts: Timing and genetic regulation of commitment to sporulation in Bacillus subtilis. Medium-dependent sporulation resulting from a mutation in the spollAB gene of Bacillus subtilis
- Abstracts: Identification and expression cloning of a leptin receptor, OB-R. Evidence that the diabetes gene encodes the leptin receptor: identification of a mutation in the leptin receptor gene in db/db mice
- Abstracts: Identification of a 29 kDa protein in the envelope of Mycobacterium smegmatis as a putative ferri-exochelin receptor
- Abstracts: Sequence analysis of a 50 kb region between spoOh and rrnH on the Bacillus subtilis chromosome. New genes in the 170 degrees region of the Bacillus subtilis genome encode DNA gyrase subunits, a thioredoxin, a xynalase and an amino acid transporter
- Abstracts: New insertion sequences and a novel repeated sequence in the genome of Mycobacterium tuberculosis H37Rv. Physical mapping of Mycobacterium bovis BCG Pasteur reveals differences from the genome map of Mycobacterium tuberculosis H37Rv and from M. bovis