Incorporation of (3H)leucine and (3H)valine into protein of freshwater bacteria: uptake kinetics and intracellular isotope dilution
Article Abstract:
The uptake kinetics, isotope dilution and incorporation of leucine and valine into proteins of freshwater bacteria in two eutrophic lakes were studied. The results showed that the uptake kinetics and isotope dilution between freshwater and marine bacteria aredifferent. However, the basic biochemical pathways of amino acid incorporation were similar in both cases. In freshwater bacteria, the incorporation of leucine and valine are closely related. These results demonstrate that measurements of leucine and valine incorporation reflects realistic values of bacterial production in lake water.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1992
User Contributions:
Comment about this article or add new information about this topic:
Effects of viruses on nutrient turnover and growth efficiency of noninfected marine bacterioplankton
Article Abstract:
The production of organic C, P and N compounds by viral lysis stimulates the growth of noninfected bacteria in marine environments suggesting that viral activity recycles nutrients in the microbial food web. The growth efficiency of noninfected bacteria decreases in the presence of viruses. The extracellular breakdown of polymeric organic nitrogen and phosphorous in cell lysates probably causes an increase in energy consumption and a decrease in growth ability. The addition of viruses to cultures with low P enhances the uptake of organic C and alkaline phosphatase activity.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1996
User Contributions:
Comment about this article or add new information about this topic:
Incorporation of (3H)leucine and (3H)valine into protein of freshwater bacteria: field applications
Article Abstract:
The applicability of leucine and valine incorporation into proteinof freshwater bacteria as a measure of bacterial production was assessed. Experiments were conducted in two eutrophic Danish lakes, and leucine and valine incorporations were compared to results of thymidine incorporation and dissolved free amino acids (DFAA) incorporation. The results showed that measurements of leucine and valine incorporation correlated well with bacterialnumbers and DFAA results. Incorporation values and bacterial carbon production were also dependent on depth.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1992
User Contributions:
Comment about this article or add new information about this topic:
- Abstracts: Distribution and diversity of natural product genes in marine and freshwater cyanobacterial cultures and genomes
- Abstracts: Heavy metals alter the electrokinetic properties of bacteria, yeasts, and clay minerals. Transformation of Bacillus subtilis by DNA bound on montmorillonite and effect of DNase on the transforming ability of bound DNA
- Abstracts: Production of monoclonal antibodies to Listeria monocytogenes and their application to determine the virulence of isolates from channel catfish
- Abstracts: Conversion of phenylalanine to benzaldehyde initiated by an aminotransferase in Lactobacillus plantarum. Application of p-toluidine in chromogenic detection of catechol and protocatechuate, diphenolic intermediates in catabolism of aromatic compounds
- Abstracts: Distribution of Aeromonas species in the intestinal tracts of river fish. Application of ozone disinfection to remove Enterococcus seriolicida, Pasteurella piscicida and Vibro anguillarium from seawater