Induction of the tod operon by trichloroethylene in Pseudomonas putida TVA8
Article Abstract:
A study was conducted to test whether trichloroethylene (TCE) influences the tod operon in the bioluminescent reporter Pseudomonas putida TVA8 using biochemical and molecular methods. The growing-cell assay was utilized to determine the bioluminescence response over time of TVA8 to different concentrations of TCE. Results indicated that specific bioluminescence from growing-cell assays can be utilized to determine enzyme activity on toluene-induced bioreactor and groundwater samples.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1998
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NAH plasmid-mediated catabolism of anthracene and phenanthrene to naphthoic acids
Article Abstract:
The napthalene plasmid degradative enzyme system is involved in the degradation of higher-molecular-weight polycyclic aromatic hydrocarbons other than napthalene. The metabolism of anthracene and phenanthrene by plasmid pKA1 is confirmed, and by using mutant strain 5RL which contains the lux reporter gene transposon (Tn 4431) in the sal operon of pKAI, the role of the NAH upper pathway operon is demonstrated.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1993
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A bioluminescent whole-cell reporter for detection of 2,4-dichlorophenoxyacetic acid and 2,4-dichlorophenol in soil
Article Abstract:
Research describes the construction of a reporter molecule for eliciting bioluminescent response from cells that receive it. Results show that when introduced on to Ralstonia eutropha JMP134 chromosome, the reporter produced bioluminescent response to herbicides.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2000
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- Abstracts: Degradation of 1,2,3,4-tetrachlorobenzene by Pseudomonas chlororaphis RW71. Chromosomal integration of tcb chlorocatechol degradation pathway genes as a means of expanding the growth substrate range of bacteria to include haloaromatics
- Abstracts: Removal of mercury from chloralkali electrolysis wastewater by a mercury-resistant Pseudomonas putida strain. Effect of oxygen on formation and structure of Azotobacter vinelandii alginate and its role in protecting nitrogenase
- Abstracts: Effect of temperature, pH, and initial cell number on luxCDABE and nah gene expression during naphthalene and salicylate catabolism in the bioreporter organism Pseudomonas putida RB1353