Influence of bovine slurry deposition on the structure of nodulating Rhizobium leguminosarum bv. viciae soil populations in a natural habitat
Article Abstract:
A modified version of the enterobacterial repetitive intergenic consensus (ERIC)-polymerase chain reaction (PCR) method is used to identify Rhizobium leguminosarum strains bv. viciae in soil from a grass-covered valley area contaminated by slurry deposition. Results indicate that the proposed method is very useful for determining the different factors that affect the structure of the nodulating R. leguminosarum bv. viciae population in a natural environment.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1996
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High-efficiency transformation of Rhizobium leguminosarum by electroporation
Article Abstract:
High-efficiency electrotransformation of Rhizobium leguminosarum has been achieved using a 15.1-kb plasmid, pMP154, having a nodABC-lacZ fusion by electroporation. The highest degree of transformation efficiency was determined to be 10(super 8) transformants/microg of DNA at a field strength of 14 kV/cm with a pulse of 7.3 ms. It was found that the number of transformants increased as cell density increased without any indication of saturation.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1999
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