Influence of metronidazole, CO, CO2, and methanogens on the fermentative metabolism and the anaerobic fungus Neocallimastix sp. strain L2
Article Abstract:
The impact of metronidazole carbonmonoxide, methanogens and carbon dioxide on the fermentation of glucose by the anaerobic fungus Neocallimatix sp. strains L2 examined indicated that metronidazole and carbon monoxide brought a shift in the fermentation products from hydrogen, acetate and formate to lactate as the major product and caused a lower glucose consumption rate. There was a heightened lactate dehydrogenase activity in cells culture under both culture conditions. Low levels of carbon dioxide gas caused an increase in hydrogen and lactate formation and reduced production of formate, acctate, succinate and ethanol, reduced glucose consumption rate and cell protein yield and also a reduction in hydrogenosomal enzyme activities.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1993
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Purification and partial characterization of two feruloyl esterases from theanaerobic fungus Neocallimastix strain MC-2
Article Abstract:
Two extracellular feruloyl esterases were isolated andpurified from the anaerobic fungus Neocallimastix strain MC-2. Partial characterization of the purified enzymes, designated FAE-I and FAE-II, showed that their molecular masses correspond to 69- and 24-kDa, respectively. The optimum pH of FAE-I is at pH 6.2, while FAE-II is most active at pH 7.0. Both enzymes displayed Michaelis-Menten kinetics, with FAE-II being specific to feruloyl esters. FAE-I, however, was able to act on both feruloyl and the equivalent para-coumaroyl esters.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1992
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P-coumaroyl and feruloyl arabinoxylans from plant cell walls as substrates from ruminal bacteria
Article Abstract:
The development of pure cultures of ruminal bacteria with various substrate propensities on particular phenolic acid-carbohydrate complexes is discussed. Ester-linked feruloyl and p-coumaroylwere used to limit the growth of Ruminococcus flavefaciens FD1, Selenomonas ruminantium HD4 and Butyrivibrio fibrisolvens 49. It was found that the hydrolization of carbohydrate linkages affected the variations in the growth limitations of phenolic acid- carbohydrate complexes.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1993
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- Abstracts: Inheritance of mitochondrial DNA and plasmids in the acomycetous fungus, Epichloe typhina. Mitochondrial DNA length variation and heteroplasmy in populations of white sturgeon (Acipenser transmontanus)
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