Inhibition of existing denitrification enzyme activity by chloramphenicol
Article Abstract:
Chloramphenicol is a bacteriostatic agent assumed to affect protein synthesis but not enzyme activity. However, chloramphenicol amended to samples of sediment and groundwater exhibiting denitrogenase enzyme activity (DEA) was shown to inhibit DEA. Differences between control and antibiotic-amended treatments were not necessarily due to new enzyme synthesis, since chloramphenicol also inhibited DEA in a third treatment where denitrification had already begun. Chloramphenicol also inhibited DEA in carbon-starved cultures of Pseudomonas denitrificans.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1992
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Effect of treated-sewage contamination upon bacterial energy charge, adenine nucleotides, and DNA content in a sandy aquifer on Cape Cod
Article Abstract:
The metabolic status of microbial communities in a contaminated plume at Cape Cod was studied using nucleic acid and adenylate indicators, which included adenylate energy charge, total adenine nucleotides, DNA levels and ATP to DNA ratios. The results showed that these indicators may be useful tools in assessing the metabolic status of free-living bacteria in contaminated aquifers. However, since the bacterial DNA content may change in response to nutrient conditions, care should be taken in using the ATP/DNA ratio in such studies.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1993
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Transport behavior of groundwater protozoa and protozoan-sized microspheres in sandy aquifer sediments
Article Abstract:
Examination of the transport behavior of groundwater flagellated protozoa and flagellate-sized carboxylated microspheres in sandy, organically polluted aquifer sediments using small-scale tracer tests reveals that the 2-micrometer flagellates have the optimum size for transport. Presence of aquifer sediments induces significant flagellate immobilization. Experimental data reveal the decrease in the immobilization rates of laboratory-grown flagellates with increasing time and distance of travel.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1995
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