Instability of a plasmid-borne inverted repeat in Saccharomyces cerevisiae

Article Abstract:

A study was conducted on the stability of a plasmid-borne 94-base pair palindromic insertion incorporated in the yeast plasmid beta-galactose gene. The inverted repeat, which had a poly GT sequence, exhibited instability in Saccharomyces cerevisiae and displayed a minimal deletion frequency of approximately 10(super -4) per mitotic division. All deletions were found to involve an interaction between two 5-base pair repeat flanking the inverted repeat. Results also indicated that a rad52 mutation did not influence both the deletion rate and the deletion type.

Analysis, Nucleotide sequence, Base sequence, Chromosome deletion

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Analysis of a gene conversion gradient at the HIS4 locus in Saccharomyces cerevisiae

Article Abstract:

An analysis of the different rates of heteroduplex formulation at the 5'and 3' ends of Saccharomyces cerevisiae's HIS4 gene shows that the direction of mismatch repair is primarily responsible for the gradient of gene conversion. Differences in the frequency of heteroduplex formulation do not determine the gradients for gene conversion (meiotic recombination). The analysis has also helped identify a site between the 5' and 3' end of the HIS4 gene, which is responsible for the formulation of heteroduplexes at HIS4 and BIK1.

Plant genetics

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Genetic evidence that the meiotic recombination hotspot at the HIS4 locus of Saccharomyces cerevisiae does not represent a site for a symmetrically processed double-strand break

Article Abstract:

Two diploid strains having multiple heterozygous palindromic oligonucleotide insertions were constructed to determine the effect of Rap1 on heteroduplex formation in BIK1 and at the 3' end of HIS4 and the direction of this effect. Results indicate that approximately 50% of heteroduplexes formed at BIK1 and and the 5' end of HIS4 are Rap1-dependent. It was also found that a majority of the recombination events induced by Rap1 binding are unidirectional, involving only either BIK1 or HIS4.

DNA, Meiosis

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• Abstracts: Isolation and characterization of SUA5, a novel gene required for normal growth in Saccharomyces cerevisiae. cis- and trans-acting suppressors of a translation initiation defect at the cyc1 locus of Saccharomyces cerevisiae

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