Integrative model for binding of Bacillus thuringiensis toxins in susceptible and resistant larvae of the diamondback moth (Plutella xylostella)
Article Abstract:
A susceptible strain of diamondback moth and resistant strains of diamondback moth from the Philippines, Hawaii and Pennsylvania were subjected to competitive binding tests. A model which includes at least four binding sites involved in binding of Cry1Aa, Cry1Ab, Cry1Ac, Cry1B, Cry1C and Cry1F was proposed. Results showed that in each of the three resistant strains, the common binding site is altered. In the strain from the Philippines, the alteration reduced binding of Cry1Ab but not of the other crystal proteins, while in the resistant strains from Hawaii and Pennsylvania, the alteration affected binding of Cry1Aa, Cry1Ab, Cry1Ac, Cry1B and Cry1F.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1999
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Evaluation of synergism among Bacillus thuringiensis toxins
Article Abstract:
It has been proposed that the Bacillus thuringiensis insecticidal toxins may interact to produce more effective insecticidal effects than single toxins. To test this hypothesis, a series of equations were developed to test synergism between toxins. Using these concepts, the CytA and CryIV toxins of B. thuringiensis subsp. israelensis and the CryIV and CryIA toxins of B. thuringiensis subsp. kurstaki were tested. The results showed that synergism exists between the CryIV and CytA toxins of B. thuringiensis subsp. israelensis. The methods developed could have a general application for testing synergistic effects in any poisons.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1992
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Resistance to toxins from Bacillus thuringiensis subsp. kurstaki causes minimal cross-resistance to B. thuringiensis subsp. aizawai in the diamondback moth (Lepidoptera: Plutellidae)
Article Abstract:
The responses of resistant diamondback moth larvae to formulations and toxins of Bacillus thuringiensis subsp. kurstaki were characterized. Resistance to B. thuringiensis subsp. kurstaki formulation conferred minimal cross-resistance to B. thuringiensis subsp. aizawai and CryIC. This cross-resistance may be caused by resistance to CryIA toxins shared by B. thuringiensis subsp. kurstaki and B. thuringiensis subsp. aizawai.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1993
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- Abstracts: Synergism between Bacillus thuringiensis spores and toxins against resistant and susceptible diamondback moths (Plutella xylostella)
- Abstracts: Binding of the CryIVD toxin of Bacillus thuringiensis subsp. israelensis to larval dipteran midgut proteins. High-level cryIVD and cytA gene expression in Bacillus thuringiensis does not require the 20 kilodalton protein, and the coexprssed gene products are synergistic in their toxicity to mosquitoes
- Abstracts: Molybdenum oxidation by Thiobacillus ferrooxidans. Isolation and characterization of a Mo6+-reducing bacterium
- Abstracts: Classification of virulent and temperate bacteriophages of Listeria spp. on the basis of morphology and protein analysis
- Abstracts: Using apomictic tetraploids to make a self-incompatible diploid Pensacola bahiagrass clone set seed. Genetics of red and purple plant color in pearl millet