Isolation and characterization of a strong promoter from the Streptomyces ghanaensis phage 119 using the gentamicin resistance gene (aacC1) of Tn1696 as reporter
Article Abstract:
A Streptomyces ghanaensis phage 119 DNA fragment that exhibited strong promoter activity was isolated by utilizing the promoterless gentamycin resistance (aacC1) gene of the Tn1696 transposon. The expression of the bifunctional pGL7011 promoter-probe shuttle plasmid in Escherichia coli and Streptomyces lividans TK23 led to the isolation of a fragment (F14) that exhibited high promoter activity in Streptomyces lividans TK23. Furthermore, the promoter element on F14 conferred high levels of kanamycin resistance in Streptomyces lividans TK23 strains.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 1997
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Sequence, locatization and characteristics of the replicator region of the symbiotic plasmid of Rhizobium etli
Article Abstract:
Nucleotide sequence analysis of the organism Rhizobium etli CFN42's symbiotic plasmid replicator region identifies three ORFs which are like those found in the replicator regions of the bacteria Agrobacterium plasmids pTiB6S3 and pRiA4b. Fragment studies identify a 5.6 kb Hindlll plasmid derivative that can reproduce on its own. The replicator sequence is seen in the other symbiotic plasmids.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 1997
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The homologous terminal sequence of the Streptomyces lividans chromosome and SLP2 plasmid
Article Abstract:
Results describe terminal sequence of a 10.1 kilobase DNA of the Streptomyces lividans chromosome sharing sequence similarity with the plasmid SLP2. Data show that a 1 kilobase stretch exhibits a stand bias in terms of guanidine and cytosine nucleotide residues.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 2000
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