Isolation and characterization of acetic acid-tolerant galactose-fermenting strains of Saccharomyces cerevisiae from a spent sulfite liquor fermentation plant

Article Abstract:

Enrichment cultures of yeast isolates obtained from spent sulfite liquor yielded two types of yeast: Saccharomyces cerevisiae and Pichia membranaefaciens. Two strains of S. cerevisiae produced more ethanol than baker's yeast at pH < 6.0 and in the presence of acetic acid. One of the S. cerevisiae strains showed catabolite repression on glucose and galactose in the absence of acetic acid. However, in the presence of acetic acid, the isolate fermented glucose and galactose simultaneously. Although none of the isolates fermented xylose, low levels of xylose reductase and xylitol dehydrogenase activities were detected.

Research, Acetic acid

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Xylose-metabolizing Saccharomyces cerevisiae strains overexpressing the TKL1 and TAL1 genes encoding the pentose phosphate pathway enzymes transketolase and transaldolase

Article Abstract:

Saccharomyces cerevisiae strains containing the Pichia stipitis XYL1 and XYL2 genes and overexpressing the TKL1 and TAL1 genes show increased growth on xylose as compared to those expressing only the XYL1 and XYL2 genes. The overexpression of only TKL1 has no effect on the bacterial growth. The rate of xylose metabolism increases with the increase in fermentation of a xylose-glucose mixture during oxygen-limited conditions. TKL1 and TAL1 encode transketolase and transaldolase, respectively, which are involved in xylose metabolism by the pentose phosphate pathway.

Enzymes, Gene expression

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Cofactor dependence in furan reduction by Saccharomyces cerevisiae in fermentation of acid-hydrolyzed lignocellulose

Article Abstract:

Two strains of Saccharomyces cerevisiae, one highly tolerant to lignocellulose hydrolysates and the other a laboratory reference strain, are investigated using batch and fed-batch fermentations of hydrolysates, as well as chemostat cultures with synthetic medium. The ability to reduce 5-hydroxy-methyl furfural is important for the development of yeast strains with increased tolerance to lignocellulosic hydrolysates.

Food preparations, not elsewhere classified, All Other Miscellaneous Food Manufacturing, Industrial Organic Chemicals, Cyclic Crude and Intermediate Manufacturing, Furan, Brewers' Yeast, Analysis, Genetic aspects, Oxidation-reduction reaction, Oxidation-reduction reactions, Cellulose, Brewer's yeast, Saccharomyces cerevisiae, Furans, Chemical properties

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