Isolation and characterization of genetically engineered gallidermin and epidermin analogs

Article Abstract:

Analogs of the tetracyclic polypeptide antibiotics gallidermin (Gdm) and epidermin (Epi) produced by an expression system involving site-specific mutagenesis with the Gdm and Epi structural genes exhibit enhanced resistance to proteolytic degradation. The S-residues responsible for thioether amino acid formation are important for the synthesis of the antibiotic analogs in Streptococcus species. Replacements of residues at positions 6, 12 and 14 on the Gdm skeleton produce analogs with increased antimicrobial action and a high resistance to proteolysis.

Antibiotics, Physiological aspects, Streptococcus, Microbial mutation, Antibiotic structure-activity relationships

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Secretion of active recombinant human tissue plasminogen activator derivatives in Escherichai coli

Article Abstract:

Researchers have created a recombinant form of tissue plasminogen activator that can be produced by E. coli with no requirement for bacterial compartment preparation or in vitro refolding. The protein is secreted into the culture supernatant and has a fibrin binding site and amidolytic activity.

Microbial genetic engineering, Tissue plasminogen activator, Recombinant proteins

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Dual role of GdmH in producer immunity and secretion of the staphylococcal lantibiotics gallidermin and epidermin

Article Abstract:

The gdmH gene of Staphylococcus gallinarum not only mediates secretion of the lantibiotics gallidermin and epidermin, it also makes the bacterium immune to gallidermin. When cloned together with the gdmT gene, it enhanced the immunity mediated by FEG immunity genes.

Bacterial toxins, Staphylococcus

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