Isolation and expression in Escherichia coli of cslA and cslB, genes coding for the condroitin sulfate-degrading enzymes chondroitinase AC and chondroitinase B, respectively, from Flavobacterium heparinum
Article Abstract:
The genes for two chondroitinases were isolated from Flavobacterium heparinum and expressed in E. coli. Chondroitinase AC has 678 residues and a molecular mass of 77,169 Da; chondroitinase B is 481 residues and 53,563 Da.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2000
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Development of a genetic system for the transfer of DNA into Flavobacterium heparinum
Article Abstract:
Research describes the construction of a conjugation/integration plasmid genetic system in Flavobacterium heparinum facilitating the study of the mechanism of enzyme degradation of glycosoaminoglycan by the bacterium.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 2001
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Isolation and expression in Escherichia coli of hepB and hepC, genes coding for the glycosaminoglycan-degrading enzymes heparinase II and heparinase III, respectively, from Flavobacterium heparinum
Article Abstract:
The genes encoding heparinases II and III (designated hepB and hepC, respectively), containing open reading frames of 2,316 and 1,980 bp, respectively, are apart from each other and from heparinase I. Expression of truncated versions of these genes reveals mature forms of heparinases II and III having 746 and 635 amino acids, respectively, and molecular weights of 84,545 and 73,135, respectively. Expressed at 37 degree C, the enzymes become insoluble and most of the heparinase III protein degraded, whereas expression at 25 degrees C makes them soluble and active.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1996
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- Abstracts: Multilocus sequence typing for comparison of veterinary and human isolates of Campylobacter jejuni. Genotyping of Campylobacter spp
- Abstracts: Absence of a putative mannose-specific phosphotransferase system enzyme IIAB component in a leucocin A-resistant strain of Listeria monocytogenes, as shown by two-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis