A homokaryotic derivative of a Phanerochaete chrysosporium strain and its use in genomic analysis of repetitive elements
Article Abstract:
Researchers illustrate the use of cultures derived from a single basidiospore of Phanerochaete chrysosporium for genetic analysis of transposons. These haploid homokaryons contain a single nuclear type, unlike the two different nuclear types in dikaryons.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2000
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Method to identify specific alleles of a Phanerochaete chrysosporium gene encoding lignin peroxidase
Article Abstract:
A protocol for identification and differentiation of allelic variants of the lignin peroxidase gene in Phanerochaete chrysosporium was developed and is presented. The method involves genomic DNA amplification by the polymerase chain reaction. The amplified genes are then probed with allele-specific oligonucleotides using the Southern blot technique. This protocol allows rapid identification of lignin peroxidase genes and alleles and is useful for classifying P. chrysosporium strains.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1992
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Isolation and purification of Pyranose 2-oxidase from Phanerochaete chrysosporium and characterization of gene structure and regulation
Article Abstract:
Study done to identify and characterize the glucose-oxidizing activity of Phanerochaete chrysosporium grown on solid substrate is reported. 13C-nuclear magnetic resonance (NMR) and 13C-labeled glucose were used to unambiguously distinguish glucose 1-oxidase (GOX) from pyranose 2-oxidase (POX). High levels of pox transcript were found under carbon-starved ligninolytic conditions but not under nutrient sufficiency and thus, this regulation pattern supports the evidence that POX has a role in lignocellulose degradation.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2004
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- Abstracts: Sequence and expression of a xylanase gene from the hyperthermophile Thermotoga sp. strain FjSS3-B.1 and characterization of the recombinant enzyme and its activity on kraft pulp
- Abstracts: Composition and biophysical properties of lipids in Xenorhabdus nematophilus and Photorhabdus luminescens, symbiotic bacteria associated with entomopathogenic nematodes