Isolation of copper biochelates from Methylosinus trichosporium OB3b and soluble methane monooxygenase mutants
Article Abstract:
An extracellular copper-binding ligand (CBL) is excreted by both strain RTR and mutant derivatives of Methylosinus trichosporium OB3b in the presence and absence of copper. This was gleaned from experiments aimed at determining the physiological aspects of the external CBLs produced by two strains of the species. The ligand is produced constitutively during periods of growth. Only when copper is bound by the CBL do wild-type cells recover CBL and internalize copper. Cells with the soluble methane monooxygenase mutant phenotype cannot reacquire the CBL or copper. These results indicate that the CBL has a role in copper assimilation by the wild-type organism.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1998
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Effect of chlorinated ethene conversion on viability and activity of Methylosinus trichosporium OB3b
Article Abstract:
A research was conducted to examine the effects of chlorinated ethene transformations on the cell viability of Methylosinus trichosporium OR3b. Culture viability decreased exponentially with the amount of substrate converted. The ratios were greater than one in chlorinated ethenes indicating cell death was the predominant toxic effect. Results indicated that the cell viability was affected via a diffusible oxidation product after the rate of killing was proportional to the number of present viable cells.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1997
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A glutathione S-transferase with activity towards cis-1,2-dichloroepoxyethane is involved in isoprene utilization by Rhodococcus sp. strain AD45
Article Abstract:
Research was conducted to examine the isolation and characterization of an isoprene-utilizing organism that dechlorinates cis-1,2-dichloroepoxyethane. The presence of a conjugate of glutathione (GSH) and 1,2-epoxyhexane was observed in cell extracts of cells exposed to 1,2-epoxyhexane which suggests that GSH is the physiological cofactor of the epoxide-transforming activity. Results demonstrate that a GSH S-transferase is involved in the metabolism of isoprene.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1998
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- Abstracts: Isolation of developmentally regulated genes from the edible mushroom Agaricus bisporus. Different temporal and spatial expression of two hydrophobin-encoding genes of the edible mushroom Agaricus bisporus
- Abstracts: Expression and secretion of barley cysteine endopeptidase B and cellobiohydrolase I in Trichoderma reesei. High yield production of a bacterial xylanase in the filamentous fungus Trichoderma reesei requires a carrier polypeptide with an intact domain structure