Isolation of the mRNA-capping enzyme and ferric-reductase-related genes from Candida albicans
Article Abstract:
The mRNA-capping enzyme (mRNA 5'-guanylyltransferase) gene was cloned from a Candida albicans genomic DNA library by functional complementation of a Saccharomyces cerevisiae ceg1-delta null mutation. This gene, CGT1, encodes a 52 kDa protein that is highly homologous to S. cerevisiae Ceg1p. CGT1 in a single-copy plasmid complemented the lethality of the S. cerevisiae ceg1-delta null mutation, and like S. cerevisiae Ceg1p, bacterially expressed Cgt1p was able to form a stable complex with the GMP moiety of GTP and to synthesize the cap structure in vitro. This indicates that CGT1 is the C. albicans MRNA 5'-guanylyltransferase gene.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 1996
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Biochemical and genetic characterization of Rbf1p, a putative transcription factor of Candida albicans
Article Abstract:
The RPG-box-binding factor 1 (Rbf1p) is a DNA-binding protein which is believed to be a transcription factor of Candida albicans based on its domain structure and predominant localization in nuclei. It has a centrally located DNA-binding domain of usual size and two glutamine-rich regions correlated with transcriptional activation of Saccharomyces cerevisiae. Disruption of the corresponding gene caused filamentous growth which suggests that Rbf1p is involved in the regulation of yeast-to-filamentous transition at the transcriptional level.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 1997
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Isolation of CaSLN1 and CaNIK1, the genes for osmosensing histidine kinase homologues, from the pathogenic fungus Candida albicans
Article Abstract:
The SLN1 gene of Candida albicans was functionally cloned employing an S. cerevisiae strain in which SLN1 expression was conditionally suppressed. Deletion analysis of the cloned gene showed that the receiver domain fo C. albicans Sln1p was not needed for rescuing SLN1-deficient S. cerevisiae strains. Unlike S. cerevisiae, a null mutation of C. albicans SLN1 was viable under regular and high osmotic conditions, but it induced a slight slowing down in growth at high osmolarity.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 1998
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