Long-term starvation survival of Yersinia ruckeri at different salinities studied by microscopical and flow cytometric methods
Article Abstract:
Three strains of Yersinia ruckeri were studied to determine the effect of salinity on growth and survival. Populations of all strains showed an ability to survive at salinities less than or equal to 20%. At 35% salinity, the survival capacity significantly decreased. Direct viable counts (DVC, viable but nonculturable cells) greater than colony-forming units were not detected. Flow cytometry was determined effective in differentiating viable and nonviable cells, as well as for demonstrating genome replication. Six genomes were detected in starved cells of Y. ruckeri.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1992
User Contributions:
Comment about this article or add new information about this topic:
Flow cytometric analysis of marine bacteria with Hoechst 33342
Article Abstract:
The utilization of 4',6-diamidino-2-phenylindole and Hoechst 33342 on paraformaldehyde-fixed seawater samples to evaluate the capacity of flow cytometric estimates of bacterial abundances is discussed. The qualitative comparison was done in terms of background fluorescence, dye specificity and the coefficient of variation for blue fluorescence of natural populations. The utility of the methods for marine microbiological studies was demonstrated.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1993
User Contributions:
Comment about this article or add new information about this topic:
Flow cytometric assessment of Escherichia coli and Salmonella typhimurium starvation-survival in seawater using rhodamine 123, propidium iodide, and oxonol
Article Abstract:
Flow cytometry is a suitable technique for quantification of heterogeneity in bacterial cultures during starvation-survival. Analysis by flow cytometry shows that single-species starved bacteria are heterogeneous populations containing various subpopulations differing in cell integrity, viability and metabolic activity.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1995
User Contributions:
Comment about this article or add new information about this topic:
- Abstracts: Enumeration and biomass estimation of bacteria in aquifer microcosm studies by flow cytometry. A flow cytometry method for rapid detection and enumeration of total bacteria in milk
- Abstracts: Lagging-strand origins of the promiscuous plasmid pMV158: physical and functional characterization. Quantitative detection of Streptococcus pneumoniae cells harbouring single or multiple copies of the gene encoding the green fluorescent protein
- Abstracts: Application of ribotyping for differentiating aeromonads isolated from clinical and environmental sources. Identification of fecal Escherichia coli from humans and animals by ribotyping
- Abstracts: Augmentation of killing of Escherichia coli O157 by combinations of lactate, ethanol, and low-pH conditions. Sensitization of Listeria monocytogenes to low pH, organic acids, and osmotic stress by ethanol
- Abstracts: Distribution of a population of Rhizobium leguminosarum bv. trifolii among different size classes of soil aggregates