Loss of heterozygosity, by mitotic gene conversion and crossing over, causes strain-specific adenine mutants in constitutive diploid Candida albicans

Article Abstract:

Research was conducted to prove that many natural isolates of the constitutive diploid organism Candida albicans yield strain-specific, recessive mutants at a reproducible frequency that is as high as a few percent of the surviving cells after being exposed to UV irradiation or other mutagens. Results demonstrate that the ADE2 locus of C. albicans CA12 is heterozygous. Findings clearly indicate that the natural heterozygosity at ADE2 forms the genetic basis for the high frequency of the specific auxotrophic phenotype in CA12 and that the loss of heterozygosity at ADE2 in C. albicans CA12 results in the generation of ade2 mutants.

Gene mutations, Gene mutation, Heterozygosis, Heterozygote, Diploidy, Crossing over (Genetics)

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WO-2, a stable aneuploid derivative of Candida albicans strain WO-1, can switch from white to opaque and form hyphae

Article Abstract:

Candida albicans strain WO-2 which is a stable aneuploid derivative of strain WO-1 can switch from white to opaque and make hyphae. The electrophoretic karyotype demonstrates that the two bands present in the parent strain WO-1 are lacking. The genetic material in these missing bands had been lost as shown in blots of karyotype producing an aneuploid strain. The low growth rate of WO-2 compared to WO-1 proves that it is haploid for a significant amount of genomic material.

Usage, Karyotypes

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Allele-specific gene targeting in Candida albicans results from heterology between alleles

Article Abstract:

When replacing genes at the PHR1 locus in Candida albicans, there is a 50-fold preference for the disrupted allele over the wild-type allele. This is caused by the presence of hisG sequences in the disrupted allele and the exogenous DNA, and by the sequence divergence between PHR1-1 and PHR1-2.

Genetic recombination

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