Lysogenic bacteriophage M1 from Selenomonas ruminantium: isolation, characterization and DNA sequence analysis of the integration site
Article Abstract:
Experiments were performed to isolate and characterize a temperate bacteriophage from Selenomonas ruminantium. Polymerase chain reaction amplification and Southern blot analysis of DNA were performed to demonstrate the presence of the bacteria in the rumen. Two major ORFs surrounding the predicted attP site were demonstrated using DNA sequencing of the attP region. The presence of conserved residues within integrate proteins of several temperate phages of different bacterial species were also observed.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 1998
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A bacteriophage of Rhodopseudomonas blastica
Article Abstract:
A bacteriophage, phi-BHG1, was obtained from a small eutrophic pond from which its host, Rhodopseudomonas blastica, was originally isolated. Phi-BHG1 had an icosahedral head of 62 nm diameter and a short 39 nm tail. It had double-stranded DNA with a size of 48 kb and a G+C content of 50.6 mol%. It adsorbed to both photosynthetically and chemoheterotropically grown R. blastica at the same rate. There were no differences in the latent and rise periods and only slight changes in the burst size.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 1996
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Bacteriophage receptors on Listeria monocytogenes cells are the N-acetyglucosamine and rhamnose substituents of teichoic acids or the peptidoglycan itself
Article Abstract:
Various approaches were utilized to study the role of teichoic acids (TA) as bacteriophage receptors among selected Listeria strains. Specific receptor structures of host cells belonging to different serovars were also determined and characterized. Findings confirmed the need for TA in general and of rhamnose and glucosamine in particular for adsorption of Listeria phage A118, which is a temperate Siphovirus, attacking predominantly serovars 1/2.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 1996
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