Mechanism of transcriptional silencing in yeast
Article Abstract:
A test on two models quantitatively for the case of SIR2-dependent silencing in budding yeast, using foreign and endogenous loci is conducted. The results contradict both models and show instead that transcriptional silencing at several URA3 transgenes, and at naturally silenced endogenous HMRa and HMLalpha mating type genes, acts downstream of gene activator protein binding to strongly reduce the occupancy of TFIIB, RNA polymerase II, and TFIIE at the silenced promoters.
Publication Name: Cell
Subject: Biological sciences
ISSN: 0092-8674
Year: 2005
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The structure of T. aquaticus DNA Polymerase lll is distinct from eukaryotic replicative DNA polymerases
Article Abstract:
A structure of Polymerase lll, which provides an opportunity for the structure-based design of new antibiotics, is presented. It is observed that though it shares the common two-metal-ion mechanism shown by all polymerases, the catalytic domain of the replicative polymerase of eubacteria is not homologous to those of archaea and eukaryotes
Publication Name: Cell
Subject: Biological sciences
ISSN: 0092-8674
Year: 2006
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The structural mechanism of translocation and helicase activity in T7 RNA polymerase
Article Abstract:
A study on structural mechanism of translocation and helicase activity in T7 RNA polymerase is presented. The results reveal that the dissociation of the product pyrophosphate (PPi) after nucleotide addition produces the protein conformational change resulting in translocations and strand separation.
Publication Name: Cell
Subject: Biological sciences
ISSN: 0092-8674
Year: 2004
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