Megavesicles implicated in the rapid transport of intracisternal aggregates across the Golgi stack

Article Abstract:

Research reveals that protein aggregates formed at low temperature by molecules of up to 400 nanometer are larger than the Golgi vesicles but are transported across the Golgi stack at a higher temperature. Data further indicate that fraction of these vesicles are enclosed in the "megavesicles" of the cisternae.

United States, Japan, Switzerland, Statistical Data Included, Physiological aspects, Protein structure

---

Bidirectional transport by distinct populations of COPI-coated vesicles

Article Abstract:

Electron microscope immunocytochemistry shows that both antegrade-directed and retrograde-directed cargo exist in COPI-coated vesicles budding from Golgi cisternae in whole cells and in a cell-free system. The findings indicate that the coats of anterograde-directed and retrograde-directed Golgi-derived vesicles are almost identical. The findings also provided an opportunity to independently cross-check the electron microscopy.

Usage, Electron microscopy

---

Coupling of coat assembly and vesicle budding to packaging of putative cargo receptors

Article Abstract:

COPI-coated vesicle budding from lipid bilayer membranes were observed to need coatomer, ARF, GTP and cytoplasmic tails of putative cargo receptors or membrane cargo proteins coming from the bilayer surface. Liposome-derived COPI-coated vesicles were found to be similar to their native counterparts on the basis of diameter, buoyant density, morphology and the need for a higher temperature for budding. These findings indicate that a bivalent interaction of coatomer with membrane-bound ARF[GTP] and with the cytoplasmic tails of cargo or putative cargo receptors is the molecular basis of COPI coated assembly.

Cell receptors, Lipid membranes, Bilayer lipid membranes

Similar abstracts:

• Abstracts: Mena, a relative of VASP and Drosophila Enabled, is implicated in the control of microfilament. Putting on the brakes: a negative regulatory function for Ena/VASP proteins in cell migration
• Abstracts: Release of ubiquitin-charged Cdc34-S(squiggle)Ub from the RING domain is essential for ubiquitination of the SCF(sup)Cdc4-bound substrate Sic1
• Abstracts: SREBP cleavage-activating protein (SCAP) is required for increased lipid synthesis in liver induced by cholesterol deprivation and insulin elevation
• Abstracts: S. typhimurium encodes an activator of Rho GTPases that induces membrane ruffling and nuclear responses in host cells

This website is not affiliated with document authors or copyright owners. This page is provided for informational purposes only. Unintentional errors are possible.